SOLID ORGAN TRANSPLANTATION

TRAX Diagnostics also offers an array of tests necessary to find the right donor for Solid organ transplantation and to monitor the post-transplant period.

CODE TEST NAME METHOD SAMPLE TYPE
TTD001 HLA ABDR DNA TYPING FOR KIDNEY /
LIVER TRANSPLANT (BOTH PATIENT & DONOR
PCR -SSOP LUMINEX RECIPIENT:-6 mL (3 mL min.) whole blood in 2 Lavender
Top (EDTA) tube OR 6 mL (3 mL min.) whole blood in 1 Yellow Top (ACD) tube.
DONOR:-6 mL (3 mL min.) whole blood in 2 Lavender Top (EDTA) tube
OR 6 mL (3 mL min.) whole blood in 1 Yellow Top (ACD) tube
TTD002 HLA DSA (DONOR SPECIFIC IgG
ANTIBODIES), CLASS I & II
Bead Based assay
(Luminex)
RECIPIENT: – 3 mL (2 mL min.) serum from 1 SST.
DONOR: -12 mL (10 mL min.) whole blood
in 2 Yellow Top (ACD) tubes
TTD003 HLA MIXED ANTIBODY SCREEN –
PRA CLASS I, II & MICA
Bead Based assay
(Luminex)
RECIPIENT:- 3 mL (1.5 mL min.) serum from 1 SST
TTD004 HLA SINGLE ANTIGEN BEAD ASSAY
FOR CLASS I IgG ANTIBODIES
Bead Based assay
(Luminex)
RECIPIENT:- 3 mL (1.5 mL min) serum from 1 SST
TTD005 HLA SINGLE ANTIGEN BEAD ASSAY FOR CLASS II IgG ANTIBODIES Bead Based assay(Luminex) RECIPIENT:- 3 mL (1.5 mL min) serum from 1 SST
TTD006 HLA SINGLE ANTIGEN BEAD ASSAY FOR CLASS I & II IgG ANTIBODIES; DSA-SAB CLASS I & II Bead Based assay
(Luminex)
RECIPIENT: – 3 mL (1.5 mL min.) serum from 1 SST
TTD007 HLA T & B CELL FLOW CYTOMETRY,
CROSS MATCH
Cell based Assay
(Flow Cytometry)
RECIPIENT:- 3 mL (1.5 mL min.) serum from 1 SST AND 8 mL
(4 mL min.) whole blood from 2 Green Top (Sodium Heparin) tubes / Yellow Top (ACD) tubes.
DONOR:- 12 mL (8 mL min.) whole blood in 3 Green Top (Sodium Heparin) tubes / Yellow Top (ACD) tubes
TTD008 HLA T & B CELL LYMPHOCYTE
CROSS MATCH
Complement mediated
cytotoxicity
RECIPIENT:- 8 mL (4 mL min.) whole blood in 2 Yellow Top (ACD) tube AND 3 mL (2 mL min.) serum from 1 SST. Collect specimen preferably 48 hours after dialysis.
DONOR:- 12 mL (10 mL min.) whole blood in 2 Yellow Top (ACD) tubes
TTD009 HLA – PANEL REACTIVE IgG
ANTIBODIES (PRA), SCREEN
Bead Based assay
(Luminex)
RECIPIENT:- 3 mL (1.5 mL min.) serum from 1 SST
TTD010 HLA – PANEL REACTIVE IgG
ANTIBODIES (PRA), CLASS I, QUANTITATIVE
Bead Based assay
(Luminex)
RECIPIENT:- 3 mL (1.5 mL min.) serum from 1 SST
TTD011 HLA – PANEL REACTIVE IgG
ANTIBODIES (PRA), CLASS II, QUANTITATIVE
Bead Based assay
(Luminex)
RECIPIENT:- 3 mL (1.5 mL min.) serum from 1 SST
TTD012 HLA DNA TYPING HIGH RESOLUTION BY
NEXT GENERATION SEQUENCING (NGS)
(For Organ Tx)
NGS RECIPIENT:- 6 mL (4 mL min.) whole blood
in 2 EDTA tube (or)
DONOR:- 6 mL (4 mL min.) whole blood in 2 EDTA tube
TTD016 DONOR DERIVED CELL FREE DNA
(dd-cfDNA) – SOT SCREENING
D-PCR (PRE) 5mL K3 EDTA / ACD Whole Blood Donor +
5mL K3 EDTA / ACD Whole Blood Recipient
TTD017 DONOR DERIVED CELL FREE DNA
(dd-cfDNA) – SOT MONITORING
D-PCR (POST TX) 10 mL Whole Blood of Recipient in 2 Streck / PAXgene
Tubes available from Trax. Gently Mix by
inverting 8-10 times
TTD018 TACROLIMUS GENETIC TEST RT-PCR (PRE TX) 5mL K3 EDTA Whole Blood Recipient

HLA DISEASE ASSOCIATION</h4

HLA – A” 2901 / 2902 for Birdshot Retinopathy
HLA – B* 27 for Ankylosing Spondylitis
HLA – B* 5 (51/52) for Behcet’s disease
HLA – B* 5701 for Abacavir sensitivity
HLA – B” 5801 for Allopurinol induced Stevens – Johnson syndrome risk
HLA – DQA” 05 / DQB1″ 02, DQA* 03 / DQB1″ 0302 for Celiace disease risk

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